PDT REDUCES GLYCOLYSIS ACTIVITY AND INDUCES CANDIDA ALBICANS DEATH

Abstract

Introduction: Candida species species can cause superficial damage to the mucosal, as well as systemic infections and is most commonly found in immunocompromised patients. The use of PDT has become promising. Objectives: To evaluate the effect of PDT doses on the bioenergetic metabolism. Methods: A suspension of C. albicans with the following experimental groups were prepared: L+P+ exposed to the LED irradiation in the presence of the photosensitizer; L+ P- exposed only LED irradiation; L-P+ only treated with the photosensitizer; L-P- without exposure to laser or photosensitizer. For determining the growth curve a suspension of C. albicans was adjusted, and inoculated in BHI. The growth was monitored in the times 0, 5, 8, 10 and 12 hours. For measurement of glucose was performed using an enzymatic glucose kit (Biotecnica). Germ tube formation was evaluated in Neubauer chamber and expressed in percentage. The conduction microcalorimeter too was evaluation. Results: The applied of PDT 15, 30 and 60 j/cm² shows differences when compared with the control group in relation the number of colony forming units. The growth kinetics was affected as well as the consumption of glucose was proportional the capacity of cell consumption, affected by treatment with PDT. The release of energy was reduced dose dependent manner to treatment. Conclusion: our results suggest that PDT cause damages on cell metabolism after generation of ROS that inhibit the activity of enzymes from biochemistry pathways, leading cell death.